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imagequant software molecular dynamics v5.0  (Molecular Dynamics Inc)

 
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    Molecular Dynamics Inc imagequant software molecular dynamics v5.0
    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with <t>ImageQuant</t> using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).
    Imagequant Software Molecular Dynamics V5.0, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+software+molecular+dynamics+v5%2E0/imagequant+software+molecular+dynamics/pmc03482212-129-24-26
    Average 90 stars, based on 1 article reviews
    imagequant software molecular dynamics v5.0 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Autoinducers Act as Biological Timers in Vibrio harveyi"

    Article Title: Autoinducers Act as Biological Timers in Vibrio harveyi

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0048310

    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).
    Figure Legend Snippet: Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).

    Techniques Used: Membrane, Incubation, Purification, Phospho-proteomics, SDS Page, Autoradiography

    Related Articles

    Software:

    Article Title: Autoinducers Act as Biological Timers in Vibrio harveyi
    Article Snippet: Gels were dried at 80°C on filter paper, exposed to a phosphoscreen for at least 24 h, and subsequently scanned using a PhosphorImagerSI (GE Healthcare). .. Different dilutions of [γ- 32 P] ATP were used to generate a calibration curve for quantification of the signal intensities of phosphorylated proteins using ImageQuant software (Molecular Dynamics V5.0; GE Healthcare). ..



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    imagequant software molecular dynamics v5.0  (Molecular Dynamics Inc)
    90
    Molecular Dynamics Inc imagequant software molecular dynamics v5.0
    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with <t>ImageQuant</t> using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).
    Imagequant Software Molecular Dynamics V5.0, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+software+molecular+dynamics+v5%2E0/imagequant+software+molecular+dynamics/pmc03482212-129-24-26
    Average 90 stars, based on 1 article reviews
    imagequant software molecular dynamics v5.0 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).

    Journal: PLoS ONE

    Article Title: Autoinducers Act as Biological Timers in Vibrio harveyi

    doi: 10.1371/journal.pone.0048310

    Figure Lengend Snippet: Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).

    Article Snippet: Different dilutions of [γ- 32 P] ATP were used to generate a calibration curve for quantification of the signal intensities of phosphorylated proteins using ImageQuant software (Molecular Dynamics V5.0; GE Healthcare).

    Techniques: Membrane, Incubation, Purification, Phospho-proteomics, SDS Page, Autoradiography