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imagequant software molecular dynamics v5.0  (Molecular Dynamics Inc)

 
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    Molecular Dynamics Inc imagequant software molecular dynamics v5.0
    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with <t>ImageQuant</t> using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).
    Imagequant Software Molecular Dynamics V5.0, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+software+molecular+dynamics+v5%2E0/pmc03482212-129-24-26?v=Molecular+Dynamics+Inc
    Average 90 stars, based on 1 article reviews
    imagequant software molecular dynamics v5.0 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Autoinducers Act as Biological Timers in Vibrio harveyi"

    Article Title: Autoinducers Act as Biological Timers in Vibrio harveyi

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0048310

    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).
    Figure Legend Snippet: Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).

    Techniques Used: Membrane, Incubation, Purification, Phospho-proteomics, SDS Page, Autoradiography



    Similar Products

    imagequant software molecular dynamics v5.0  (Molecular Dynamics Inc)
    90
    Molecular Dynamics Inc imagequant software molecular dynamics v5.0
    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with <t>ImageQuant</t> using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).
    Imagequant Software Molecular Dynamics V5.0, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+software+molecular+dynamics+v5%2E0/pmc03482212-129-24-26?v=Molecular+Dynamics+Inc
    Average 90 stars, based on 1 article reviews
    imagequant software molecular dynamics v5.0 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).

    Journal: PLoS ONE

    Article Title: Autoinducers Act as Biological Timers in Vibrio harveyi

    doi: 10.1371/journal.pone.0048310

    Figure Lengend Snippet: Inverted membrane vesicles prepared from E. coli TKR2000 containing full-length LuxQ were incubated with purified LuxP, purified LuxU and (where indicated) with 10 μM AI-2 (A). The phosphorylation reaction was started by adding 100 μM [γ- 32 P] ATP at time 0. At the indicated times, the reaction was terminated, and radiolabeled proteins were separated by SDS-PAGE, and visualized by autoradiography. The arrow indicates phosphorylated LuxU. Phosphorylated LuxU was quantified with ImageQuant using [γ- 32 P] ATP as standard (B). Phosphorylation experiments were also performed in the presence or absence of 10 μM HAI-1 using membrane vesicles containing full-length LuxN and phosphorylated LuxU was quantified accordingly (B).

    Article Snippet: Different dilutions of [γ- 32 P] ATP were used to generate a calibration curve for quantification of the signal intensities of phosphorylated proteins using ImageQuant software (Molecular Dynamics V5.0; GE Healthcare).

    Techniques: Membrane, Incubation, Purification, Phospho-proteomics, SDS Page, Autoradiography